Stable Isotope Probing Analysis of Interactions between Ammonia Oxidizers

Interactions between soil methane and ammonia oxidizers

Introduction Conclusions References

Distribution of ammonia oxidizers

Biogeosciences Discussions This discussion paper is/has been under review for the journal Biogeosciences (BG). Please refer to the corresponding final paper in BG if available. Abstract Nitrogen is the major limiting nutrient in cold environments, and its availability is strongly dependent on nitrification. However, microbial communities driving this process remain largely uncharacterized in al...

Stable isotope probing and dynamic loading experiments provide insight into the ecophysiology of novel ammonia oxidizers in rapid gravity sand filters

Stable isotope probing: Technical considerations when resolving

11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound. N-RNA-SIP is a new method that has been discussed in recent literature but has not yet been tested. Herein, we define the limitation...

DNA stable-isotope probing (DNA-SIP).

DNA stable-isotope probing (DNA-SIP) is a powerful technique for identifying active microorganisms that assimilate particular carbon substrates and nutrients into cellular biomass. As such, this cultivation-independent technique has been an important methodology for assigning metabolic function to the diverse communities inhabiting a wide range of terrestrial and aquatic environments. Following...